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Five new Listeria species have been described through a collaborative project between Cornell University and Mérieux NutriSciences. Three of these species have been classified into the Listeria sensu stricto clade, the clade that includes the well-known food pathogen Listeria monocytogenes (LM) and the species showing close similarity to LM. The non-LM sensu stricto species (L. innocua, L. ivanovii, L. seeligeri, L. welshimeri) play a relevant role in public health as they have shown to be good indicator or even index organisms of LM contamination.
A Technical Backstory:
Prior to this project, most of the recently described species (14/15) are so divergent from LM they are not considered relevant by industry. However, with this discovery, there are three additions to the LM indicator set of Listeria (L. cossartiae, L. farberi, and L. immobilis) based on their phenotypic and genetic resemblance to L. marthii, L. innocua, and L. ivanovii. This is the first time since 2010 a newly described species has been added to the sensu stricto clade.
What does all of that mean?
Three of the recently described species can be grouped together with those traditional indicators for potential Listeria monocytogenes contamination. None of these new species are pathogenic nor do they harbor any sanitizer resistance genes. However, given that classical confirmation methods (e.g., FDA BAM, Health Canada, ISO, USDA MLG) do not describe these species, combined with the fact rapid methods have not been verified for their inclusivity, there is potential for a false negative or misclassification as a false positive.
The key benefits of this discovery to the industry include:
How is Merieux NutriSciences addressing this discovery?
Confirmation procedures are being updated to address possible misclassification and prevent false negatives. Inclusivity experiments are being performed to verify the recently described species will be detected by the Listeria spp. rapid methods used by Merieux NutriSciences.
To learn more about these 5 novel Listeria species, please check out the free article by Mérieux Nutriscience’s Catharine Carlin published in the International Journal of Systematic and Evolutionary Microbiology. Contact us today to learn more.